Human IgG (H&L) Antibody Biotin Conjugated

Specifications

Product Data
Product Name	Human IgG (H&L) Antibody Biotin Conjugated
Applications	ELISA, IHC, WB
Recommend Dilution	WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:20,000 - 1:100,000
Host	Sheep
Immunogen	Human IgG whole molecule
Formulation	0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	2.0 mg/mL - lot specific
Conjugation	Biotin
Storage Condition	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Note	Anti-Human IgG (H&L) Biotin Conjugated Antibody has been tested by ELISA and is assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:21,000 to 1:144,000 of the reconstitution concentration is suggested for this product.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.