Dog IgG F(c) Antibody Peroxidase Conjugated

Specifications

Product Data
Product Name	Dog IgG F(c) Antibody Peroxidase Conjugated
Applications	ELISA, IHC, WB
Recommend Dilution	WB: 1:1,000 - 1:10,000 IHC: 1:500 - 1:2,500 ELISA: 1:10,000 - 1:50,000
Host	Goat
Immunogen	Anti-Dog IgG F(c) whole molecule was produced by repeated immunization with Dog IgG F(c) whole molecule in goat.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	2.0 mg/mL - lot specific
Conjugation	HRP
Storage Condition	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Note	Anti-Dog IgG F(c) Peroxidase Conjugated Antibody has been tested by ELISA. This secondary antibody anti-Dog is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:6,000 to 1:40,000 of the reconstitution concentration is suggested for this product.

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