Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody
Applications	IF
Recommend Dilution	Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 106 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications. Recommended Dilutions: Immunofluorescence: 1/100-1/250. Flow Cytometry: 1/100-1/250.
Reactivity	Mouse
Host	Goat
Immunogen	Human IgG F(c) fragment.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 Label: PE State: Lyophilized F(ab')2 fragments Stabilizer: 10 mg/ml BSA (IgG and Protease free) Preservative: 0.01% (w/v) Sodium Azide Label: R-Phycoerythrin ( (Molecular Weight 240,000 daltons) Absorption emission: 488 nm / 575 nm
Concentration	lot specific
Purification	Immunoaffinity Chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation	PE
Storage Condition	This antibody is stable at 2-8°C as an undiluted liquid. DO NOT FREEZE! Restore with 1.0 ml of deionized water (or equivalent). Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. Dilute only prior to immediate use. Avoid repeated freezing and thawing.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.