Rat IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody

Specifications

Product Data
Product Name	Rat IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/2,000-1/10,000. Western Blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500-1/2,000.
Reactivity	Rat
Host	Rabbit
Immunogen	Rat IgG whole molecule.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative. Label: HRP State: Lyophilized purified Ig fraction. Label: Horseradish Peroxidase Conjugation Chemistry: Periodate. Label MW: 40,000
Concentration	lot specific
Purification	Immunoaffinity Chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation	HRP
Storage Condition	Store vial at 2-8°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing. Dilute only prior to immediate use.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.