Human IgG (F(ab)2 specific), adsorbed Rabbit Polyclonal Antibody

Specifications

Product Data
Product Name	Human IgG (F(ab)2 specific), adsorbed Rabbit Polyclonal Antibody
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:2,500 to 1:11,000 of the stated concentration is suggested for this product.
Reactivity	Human
Host	Rabbit
Immunogen	Human IgG F(ab')2 fragment.
Formulation	0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: AP State: Liquid (sterile filtered) purified IgG fraction. Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Concentration	lot specific
Purification	Immunoaffinity chromatography.
Conjugation	AP
Storage Condition	Store the antibody at 2-8°C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Dilute only prior to immediate use.

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