Human IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody

Specifications

Product Data
Product Name	Human IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/50,000-1/100,000. Western blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500-1/2,500. Note: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:9,000 to 1:41,000 is suggested for this product.
Reactivity	Human
Host	Rabbit
Immunogen	Human IgG whole molecule.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Label: HRP State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (IgG and Protease free) Preservative: 0.01% (w/v) Gentamicin Sulfate Label: Horseradish Peroxidase
Concentration	2.0 mg/ml (by UV absorbance at 280 nm)
Purification	Immunoaffinity Chromatography
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.