Porcine IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: AP33087HR-N

Porcine IgG (H+L chain) rabbit polyclonal antibody, HRP

Conjugation: Biotin FITC HRP TRITC



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CNY 3,996.00


货期*
5周

规格
    • 1 ml

Product images

Specifications

Product Data
Product Name Porcine IgG (H+L chain) Rabbit Polyclonal Antibody
Applications ELISA, IF, IHC, WB
Recommend Dilution ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.

Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in swine serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electrondense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000.
Reactivity Porcine
Host Rabbit
Immunogen Purified normal IgG isolated from pooled Swine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Formulation PBS, pH 7.2
Label: HRP
State: Lyophilized purified hyperimmune IgG fraction
Stabilizer: None
Preservative: None, as it may interfere with the antibody activity.
Label: Horseradish peroxidase enriched for isoenzyme C (RZ = 3.2)
Molar radio: Peroxidase/IgG ~1.7
Concentration 10.0 mg/ml
Purification Hyperimmune antisera with strong activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
Conjugation HRP
Storage Condition Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Note Adsorption: Not required.
Conjugation procedure: Conjugation is carried out using a proprietary modification of the periodate method, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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