Sheep IgM (H+L chain) Rabbit Polyclonal Antibody

CAT#: AP33084AF-N

Sheep IgM (H+L chain) rabbit polyclonal antibody, Azide Free



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CNY 4,354.00


货期*
5周

规格
    • 10 mg

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Specifications

Product Data
Product Name Sheep IgM (H+L chain) Rabbit Polyclonal Antibody
Applications ELISA, IF, WB
Recommend Dilution ELISA.
Dot blot.
Immunoblotting.
Indirect Immunofluorescence.

Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Precipitin titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Reactivity Sheep
Host Rabbit
Immunogen Purified normal IgM isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Formulation PBS, pH 7.2
State: Azide Free
State: Lyophilized purified hyperimmune IgG fraction
Stabilizer: None
Preservative: None, as it may interfere with the antibody activity.
Concentration 10.0 mg/ml
Purification Hyperimmune antisera with strong activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
Conjugation Unconjugated
Storage Condition Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Note Adsorption: Not required.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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