Duck IgG (H+L chain) Rabbit Polyclonal Antibody
CAT#: AP33057BT-N
Duck IgG (H+L chain) rabbit polyclonal antibody, Biotin
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CNY 3,996.00
货期*
5周
规格
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Specifications
Product Data | |
Product Name | Duck IgG (H+L chain) Rabbit Polyclonal Antibody |
Applications | ELISA, IF, IHC, WB |
Recommend Dilution | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. In immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of duck origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/10-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/2,000-1/10,000. |
Reactivity | Duck |
Host | Rabbit |
Immunogen | Purified normal Ig fractions isolated from pooled Duck serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Formulation | PBS, pH 7.2 Label: Biotin State: Lyophilized purified hyperimmune IgG fraction Stabilizer: None Preservative: None Label: N-Hydroxysuccinimidobiotin Molar radio: Biotin/IgG protein ~4.6 |
Concentration | 10.0 mg/ml |
Purification | Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography. |
Conjugation | Biotin |
Storage Condition | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Note | Adsorption: Not required. Conjugation procedure: A proprietary technique for the binding to biotin is used, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life. |
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