Mouse IgM (Fc specific) Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Mouse IgM (Fc specific) Goat Polyclonal Antibody
Applications	ID, IF, IHC, IP
Recommend Dilution	Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of Mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working Dilutions: For histochemical and Cytochemical use are usually between 1/100 and 1/250 In ELISA and comparable non-precipitating antibody-binding assays between 1/1500 and 1/6000.
Reactivity	Mouse
Host	Goat
Immunogen	Purified homogenous IgM isolated from Mouse serum. Immunization with intact (19S) and split IgM (7S). Freund’s complete adjuvant is used in the first step of the immunization procedure.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins added. Label: Biotin State: Lyophilized purified hyperimmune Ig fraction Molar radio: 7.3
Concentration	10 mg/ml
Purification	DEAE-Column Chromatography
Conjugation	Biotin
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

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