Monkey IgM (Fc specific) Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Monkey IgM (Fc specific) Goat Polyclonal Antibody
Applications	ELISA, ID, IF, IHC, IP
Recommend Dilution	Can be used for direct immunofluorescence staining of cytoplasmic IgM of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1/10 and 1/40.
Reactivity	Monkey
Host	Goat
Immunogen	Purified polyclonal IgM and pools of homogenous IgM isolated from pooled monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Formulation	PBS, pH 7.2 No preservative added, as it may interfere with the antibody activity. No foreign proteins added. Label: TRITC State: Lyophilised hyperimmune Ig fraction Label: Tetramethylrhodamine isothiocyanate isomer R Absorption emission: 554 nm / 573 nm Molar radio: 1,3
Concentration	10 mg/ml
Purification	DEAE-column Chromatography
Conjugation	TRITC
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

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