Monkey IgM (Fc specific) Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Monkey IgM (Fc specific) Goat Polyclonal Antibody
Applications	ELISA, ID, IF, IP, WB
Recommend Dilution	As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: In Histochemistry are usually between 1/100 and 1/500. In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/5000.
Reactivity	Monkey
Host	Goat
Immunogen	Purified homogenous IgM isolated from monkey serum. Immunization with intact (19S) and split IgM (7S). Freund’s complete adjuvant is used in the first step of the immunization procedure.
Formulation	PBS, pH 7.2 No preservative added, as it may interfere with the antibody activity. No foreign proteins added. State: Azide Free State: Lyophilised hyperimmune Ig fraction
Concentration	10 mg/ml
Purification	DEAE-column Chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies crossreacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Antibody titre: Precipitin titre not less than 1/64 when tested against pooled normal monkey serum in agar-block immunodiffusion titration.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.