Rat IgE (Fc specific) Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Rat IgE (Fc specific) Goat Polyclonal Antibody
Applications	ELISA, ID, IF, IP, WB
Recommend Dilution	This antibody can be used: • As unlabelled secondary antibody for indirect detection of IgE in Rat cell, tissue substrates and body fluids. • In Immunofluorescence and immunoenzyme assay methods. • For the production of immunoconjugates with a selected marker. • To prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier. • As catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in rat serum or other body fluid. Recommended Dilutions: Histochemistry and Cytochemistry: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Reactivity	Rat
Host	Goat
Immunogen	Purified homogenous IgE isolated from Rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Formulation	PBS, pH 7.2 without preservatives. State: Azide Free State: Lyophilized purified IgG fraction.
Concentration	10 mg/ml
Purification	Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. Special attention is given to the elimination of antibodies to the common Fab portion of immunoglobulins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

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