Mouse IgE (Fc specific) Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Mouse IgE (Fc specific) Goat Polyclonal Antibody
Applications	ID, IF, IHC, IP, WB
Recommend Dilution	Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2,000.
Reactivity	Mouse
Host	Goat
Immunogen	Purified homogenous IgE isolated from Mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Formulation	PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized purified IgG fraction. Molar radio: Biotin/IgG ~ 5.5
Concentration	10 mg/ml
Purification	Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid Repeated thawing and freezing.
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

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