Human IgM (heavy chain) Mouse Monoclonal Antibody [Clone ID: SPM557]

Specifications

Product Data
Product Name	Human IgM (heavy chain) Mouse Monoclonal Antibody [Clone ID: SPM557]
Clone Name	SPM557
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivity	Human
Host	Mouse
Immunogen	Recombinant heavy chain of human IgM.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.

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