Human IgG4 Mouse Monoclonal Antibody [Clone ID: MRQ-44]

Specifications

Product Data
Product Name	Human IgG4 Mouse Monoclonal Antibody [Clone ID: MRQ-44]
Clone Name	MRQ-44
Applications	IHC
Recommend Dilution	Immunohistochemistry on Frozen and Formalin-Fixed, Paraffin-Embedded Sections: 1/100-1/500.  Preparation and Pretreatment: 1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C. 2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water. 3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step. Positive Control: Tonsil.
Reactivity	Human
Host	Mouse
Immunogen	Synthetic Human IgG4
Formulation	Tris Buffer, pH 7.3-7.7 State: Purified State: Liquid purified Tissue Culture Supernatant. Stabilizer: 1% BSA Preservative: < 0.1% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!

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