Vimentin (VIM) Biotinylated Mouse Monoclonal Detection Antibody (Biotin conjugated) [Clone ID: OTI7B10]

Specifications

Product Data
Clone Name	OTI7B10
Applications	ELISA
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human VIM (NP_003371) produced in E.coli.
Formulation	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration	0.5mg/ml
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Biotin
Predicted Protein Size	53.5 kDa
Gene Name	vimentin
Database Link	NP_003371 Entrez Gene 7431 Human UniProt ID P08670
Background	Vimentin (VIM) is a structural protein that in humans is encoded by the VIM gene. A vimentin monomer has a central α-helical domain, capped on each end by non-helical amino (head) and carboxyl (tail) domains. Two monomers are likely co-translationally expressed in a way that facilitates their formation of a coiled-coil dimer, which is the basic subunit of vimentin assembly. It is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. Intermediate filaments, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. Vimentin is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. Since vimentin is the major cytoskeletal component of mesenchymal cells, it is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition during both normal development and metastatic progression. Vimentin has been used as an immunohistochemical tumor marker of sarcoma, renal cell carcinoma, endometrial cancer, lung carcinoma, lymphoma, leukemia, and melanoma.
Reference Data
Protein Families	ES Cell Differentiation/IPS

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