mIgG (1,2a,2b,3) Rabbit Monoclonal Antibody [Clone ID: OTIR12F3]

Specifications

Product Data
Clone Name	OTIR12F3
Applications	ELISA, WB
Recommend Dilution	WB 1:2000, ELISA 1:5000-1:20000
Reactivity	Mouse
Host	Rabbit
Clonality	Monoclonal
Immunogen	Native protein
Formulation	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration	1 mg/ml
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
Predicted Protein Size	25 kD
Background	Immunoglobulin G (IgG) is the predominant antibody class present in mouse and human serum. It is also the main class used in development of antibody therapies, especially those for cancer.1 IgG is also used to treat immune defificiencies and autoimmune diseases in the form of intravenous immunoglobulin (IVIg), generated from a plasma pool of thousands of donors.Human IgG can be divided in 4 subclasses, IgG1, IgG2, IgG3 and IgG4, and mouse in IgG1, IgG2a, IgG2b and IgG3, with IgG2c being the equivalent of IgG2a in some mouse strains such as C47Bl/6 mice. All subclasses mediate effector functions slightly different due to variable specificity and affinity for the different IgG binding partners mentioned above. Most of the currently approved therapeutic monoclonal antibodies are IgG1, but also IgG2 and IgG4 are used. IgG2 and IgG4 are preferred when Fc-mediated effector functions are not needed or not preferred, as these subclasses bind with lower affinity to the FcgR and activate complement less actively.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.