CXCL8 Rabbit Monoclonal Antibody [Clone ID: WS-4]

Specifications

Product Data
Clone Name	WS-4
Applications	ELISA, IHC, Neutralize
Reactivity	Human, Rabbit
Modifications	This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.
Host	Rabbit
Clonality	Monoclonal
Immunogen	The original antibody was generated by immunizing BALB/c mice with human IL-8. This reshaped WS-4 antibody was generated by grafting CDRs of mouse monoclonal antibody onto human framework regions.
Specificity	This antibody binds human IL-8, which is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. This antibody cross reacts with rabbit IL-8. This antibody was used in the generation of a very sensitive ELISA for the detection of human IL-8 in various body fluids. The original mouse version of this antibody has a detection limit of 16 pg/ml in an ELISA. This antibody can bind both the 72 and 77 amino acid forms of IL-8 produced by cultured human cells and cell lines stimulated with IL-1 (PMID: 1593135). The neutralization activity of this antibody towards IL-8 was studied in a rabbit model of severe acute pancreatitis. It was reported that this antibody can reduce the acute lung injury, presumably through inhibition of circulating IL-8 and TNF-alpha, and CD11b/CD18 in lung tissue (PMID: 10189850). This antibody can neutralize rabbit IL-8 in vivo (PMID: 8347559). The effect of neutralizing antibody on IL-8 enhanced infectious cytomegalovirus (CMV) production was examined and it was reported that this antibody reduced virus production nearly to unstimulated levels at 1,000 ng/ml (PMID: 7933146). This antibody was used in the immunohistochemical staining and immuno-localization of IL-8 in tissue sections taken from intestinal loops of patients infected with either M90T or BS176 strains of Shigella flexneri (PMID: 10024597).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Database Link	UniProt ID P10145
Synonyms	C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; CXCL8; Emoctakin; GCP-1; Granulocyte chemotactic protein 1; IL8; Interleukin-8; MDNCF; MONAP; Monocyte-derived neutrophil-activating peptide; Monocyte-derived neutrophil chemotactic factor; NAP-1; Neutrophil-activating protein 1; Protein 3-10C; T-cell chemotactic factor
Reference Data

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