rpmH Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 0.5 μg/mL. Antibody validated: Western Blot in E.coli samples. All other applications and species not yet tested.
Reactivity	Escherichia coli
Host	Rabbit
Clonality	Polyclonal
Immunogen	Anti-rpmH/L34 antibody (10065) was raised against an 15 amino acid peptide near the carboxyl terminus of E. coli rpmH/L34. The immunogen is located within the last 30 amino acids of rpmH/L34.
Formulation	rpmH/L34 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Purification	rpmH/L34 Antibody is affinity chromatography.
Conjugation	Unconjugated
Storage Condition	rpmH/L34 Antibody can be stored at 4 °C for three months and -20 °C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Predicted Protein Size	Predicted: 5.4 kD Observed: 6 kD
Database Link	UniProt ID P0A7P5
Background	The rpmH/L34 protein is a component of the 50S subunit of the ribosome. rpmH/L34 can be isolated in a complex with 5S rRNA, tRNA, and other proteins of the large ribosomal subunit. rpmH/L34 is one of six proteins that is completely lost from ribosomes in post-stationary cells. rpmH/L34 was identified as "antizyme 2”, an inhibitor of the biosynthetic ornithine and arginine decarboxylases, enzymes are involved in the biosynthesis of polyamines.
Note	Optimal dilutions for each application to be determined by the researcher.
Reference Data

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