Cd68 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1:1000 ELISA: 1:10,000 - 1:50,000
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	CD68 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a near C-terminal region of mouse CD68.
Specificity	Anti-CD68 Antibody is directed against mouse CD68 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse based on 100% homology for the immunogen sequence. Cross reactivity with Cd68 from other sources has not been determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.02 mg/ml - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Gene Name	CD68 antigen
Database Link	Entrez Gene 12514 Mouse UniProt ID P31996
Background	CD68, cluster of differentiation, is a 110-kD transmembrane glycoprotein that is highly expressed by monocytes and tissue macrophages. CD68 is a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. Immunohistochemistry can be used to identify the presence of CD68, which is found in the cytoplasmic granules of a range of different blood cells. It is particularly useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts. This allows it to be used to distinguish diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukemia (the latter being CD68 negative). Its presence in macrophages also makes it useful in diagnosing conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease. Anti-Cd68 antibody is suitable for researchers interested in cancer research and the immune system.
Synonyms	Rabbit Anti-CD68 Antibody, Rabbit Anti-Macrosialin Antibody, Gp110, CD68, CD68 Molecule, CD68 Antigen, Scavenger Receptor Class D Member, Macrophage Antigen CD68, SCARD1, LAMP4, Macrosialin
Note	Anti-CD68 Antibody has been tested in ELISA and Western Blot. Expect a band at ~34.8Da in western blot using appropriate tissues and lysates. Positive control used: mouse heart and kidney in WB.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.