BLM Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	WB: 1ug/ml IHC: User Optimized ELISA: 1: 10,000
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Anti-BLM pS1290 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human Bloom syndrome protein.
Specificity	Anti-BLM pS1290 was affinity purified from monospecific antiserum by immunoaffinity chromatography and is directed against the phosphorylated form of human S1290 residue. A BLAST analysis was used to suggest cross-reactivity with human and mouse based on 100% sequence homology. Cross-reactivity with BLM pS1290 from other sources has not been determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration	1.1mg/mL - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Database Link	UniProt ID P54132
Background	BLM pS1290 product is a member of the RecQ helicase protein family. The protein contains 1417 amino acids; including one ATP binding site, one DEAH box, and two putative nuclear localization signals, as well as phosphorylation sites. BLS protein is known to associate and be phosphorylated by the ATR (ataxia telangiectasia, rad3+) protein, in response to genetic stress. The Bloom's syndrome gene product is thought to play a role in the etiology of two major human health problems, each of which is a very common complication of Bloom's syndrome, namely cancer and diabetes. Anti-BLM pS1290 antibody is ideal for researchers interested in Epigenetics and Nuclear Signaling or DNA Damage & Repair research.
Synonyms	rabbit anti-BLM pS1290 Antibody, DNA helicase, RecQ-like type 2, RECQ2, RECQL3, Bloom syndrome protein
Note	Anti-BLM pS1290 antibody is useful for ELISA, Immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~159kDa corresponding to the appropriate cell lysate or extract.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.