H3C14 Rabbit Polyclonal Antibody

CAT#: TA397493

Histone H3 K18ac Antibody



Need it in bulk or conjugated?
Get a free quote

CNY 8,363.00


货期*
4周

规格
    • 50 ug

Product images

经常一起买 (2)
beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control
    • 30 ul

CNY 300.00
CNY 1,430.00


Transient overexpression lysate of histone cluster 2, H3a (HIST2H3A)
    • 100 ug

CNY 3,080.00

Specifications

Product Data
Applications ChIP, IF, IHC, WB
Recommend Dilution WB: 1:500
IHC: 1:500
IF: 1:500
CHiP: 2-5µg/million cells
Reactivity C. elegans, Human, Mouse
Host Rabbit
Clonality Polyclonal
Immunogen Histone H3 [ac Lys18] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 18 of human Histone H3.2.
Specificity Anti-Histone H3 [ac Lys18] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration 1.1 mg/mL - lot specific
Conjugation Unconjugated
Storage Condition Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Gene Name histone cluster 2, H3c
Background Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Specifically, the acetylation of lysine 18 on histone 3 (H3 K18ac) is associated with transcriptional activation, DNA replication, and DNA repair. Enzymes known to acetylate K18 included Gcn5, p300, CBP, and TFIIIC90. Acetylation of this amino acid is also known to potentiate other arginine methylation sites on H3. Anti-Histone H3 are ideal for researchers interested in Chromatin Research, Epigenetics, Chromatin Modifiers, Histones and Modified Histones.
Synonyms rabbit anti-Histone H3 Ac Lys18 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87782, MGC87783, H3K18ac
Note Anti-Histone H3 [ac Lys18] antibody is tested for Western Blot, Chromatin Immunoprecipitation, Dot Blot, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
Reference Data
Protein Pathways Systemic lupus erythematosus
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...