H3C14 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ChIP, IF, IHC, WB
Recommend Dilution	WB: 1:500 IHC: 1:100 IF: 1:100 CHiP: 2-5µg/million cells
Reactivity	C. elegans, Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Histone H3 [Asym-dimethyl Arg17] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic asymmetric dimethylated peptide surrounding Arginine 17 of human Histone H3.2.
Specificity	Anti-Histone H3 [Asym-dimethyl Arg17] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	0.83 mg/ml - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Gene Name	histone cluster 2, H3c
Database Link	Entrez Gene 333932 HumanEntrez Gene 126961 Human UniProt ID Q71DI3
Background	Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, dimetylation of H3 Arg17 (H3 R17Me2) has been linked to gene activation. Coactivator-associated arginine methyltransferase-1 (CARM1) methylates Arg17 with its protein arginine methyltransferase (PRMT) catalytic core. Activation of this modification is linked to transcription hormone response promotors, as well as cell fate regulation. Interestingly, H3 methylation of R17 and R26 contributes to greater pluripotency potential of stem cells, while downregulation of this PTM increases differentiation. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
Synonyms	rabbit anti-Histone H3 Asym-dimethyl Arg17 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3R17me2a
Note	Anti-Histone H3 [Asym-dimethyl Arg17] antibody is tested for Western Blot, Chromatin Immunoprecipitation, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
Reference Data
Protein Pathways	Systemic lupus erythematosus

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