Pax7 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1.0 ug/ml ELISA: 1:10,000 - 1:40:000
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Pax7 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Pax7 protein.
Specificity	Anti-Pax-7 was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross reactivity with Pax7 protein from mouse (100% homology) and human (66.7% homology) for the immunizing sequence. Cross-reactivity with Pax7 from other sources has not been determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/ml - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Gene Name	paired box 7
Database Link	Entrez Gene 18509 Mouse UniProt ID P47239
Background	The Anti-Pax7 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Paired box protein Pax-7 transcription factor plays a role in myogenesis through regulation of muscle precursor cells proliferation. These genes play critical roles during fetal development and cancer growth. It is expressed specifically during the development of the nervous and muscular system. Anti-Pax7 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.
Synonyms	rabbit anti-Pax7 antibody, Paired box protein Pax-7, Pax 7, Pax-7
Note	Anti-Pax7 antibody has been tested in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 54.9 kDa in size corresponding to Pax7 by western blotting in the appropriate cell lysate or extract.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.