MDH1 Sheep Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	WB: 1:500 - 1:5,000 ELISA: 1:5,000 - 1:20,000
Reactivity	Porcine
Host	Sheep
Clonality	Polyclonal
Immunogen	Malate Dehydrogenase [Pig Heart]
Specificity	Anti-MALATE DEHYDROGENASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart].  Cross reactivity against Malate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration	1.0 mg/ml - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Database Link	UniProt ID P11708
Background	Catalytic activity of Malate Dehydrogenase is (S)-malate + NAD+ = oxaloacetate + NADH.
Synonyms	sheep anti-Malate Dehydrogenase Antibody, Malate dehydrogenase, cytoplasmic, Cytosolic malate dehydrogenase
Note	MALATE DEHYDROGENASE antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Malate Dehydrogenase [Pig Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
Reference Data

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