Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1:1000 ELISA: 1:20,000 - 1:100,000
Reactivity	Streptomyces avidinii
Host	Rabbit
Clonality	Polyclonal
Immunogen	Streptavidin [Streptomyces avidinii]
Specificity	Streptavidin Antibody Peroxidase Conjugate is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Streptavidin [Streptomyces avidinii].  No cross reactivity occurs against avidin.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 2.0 mL
Concentration	10.0 mg/mL - lot specific
Conjugation	HRP
Storage Condition	Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Background	Anti-streptavidin antibody detects Streptavidin, a 60 kDa protein purified from the bacterium Streptomyces avidinii. Streptavidin is a homo-tetrameric protein having a very high affinity for biotin (vitamin B7). Streptavidin bound biotin has a dissociation constant (Kd) on the order of about 10^−14 mol/L. Streptavidin and biotin are used extensively in molecular biology and bionanotechnology due to the resistance of streptavidin-biotin complex to organic solvents, denaturants (e.g. guanidinium chloride), detergents (e.g. SDS, Triton), proteolytic enzymes, and extremes of temperature and pH.
Synonyms	rabbit anti-Streptavidin Antibody HRP Conjugation, Peroxidase conjugated rabbit anti-Streptavidin antibody, Anti-Streptavidin HRP Antibody
Note	Anti-Streptavidin Peroxidase Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Streptavidin in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) for 30 minutes at room temperature. Optimal concentrations in immunoassays should be determined by researcher.
Reference Data

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