ampC Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IP, WB
Recommend Dilution	WB: 1:500 - 1:5,000
Reactivity	Enterobacter cloacae
Host	Rabbit
Clonality	Polyclonal
Immunogen	Penicillinase [E.cloacae]
Specificity	Anti-Penicillinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Penicillinase [E.cloacae].  Cross reactivity against Penicillinase from other tissues and species may occur but have not been specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/ml - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	UniProt ID P05364
Background	Beta-lactamase or Penicillinase has catalytic activity of a beta-lactam + H2O = a substituted beta-amino acid.
Synonyms	rabbit anti-Penicillinase Antibody, Aer1 antibody, Beta lactamase 1 antibody, Beta lactamase AER 1 antibody, OXA9 antibody, Oxacillinase antibody, PenPC antibody, Cephalosporinase
Note	Penicillinase Antibody has been tested by western blot and is suitable to be assayed against 1.0 µg of Penicillinase [E.cloacae] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:7,000 to 1:21,000 of the reconstitution concentration is suggested for this product.
Reference Data

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