H3C14 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ChIP, ELISA, IF, IHC, IP, WB
Recommend Dilution	WB: 1:500 - 1:2,000 IHC: 2 - 5 µg/ml IF: 2 - 5 µg/ml CHiP: 2-5µg/million cells ELISA: 1:3,000 - 1:10,000
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Histone H3 K4me1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 4 of human Histone H3.2.
Specificity	Anti-Histone H3 [Monomethyl Lys4] was purified from monospecific antiserum by protein A affinity purification. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Gene Name	histone cluster 2, H3c
Database Link	Entrez Gene 126961 Human UniProt ID Q71DI3
Background	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics Research.
Synonyms	rabbit anti-Anti-Histone H3 Monomethyl Lys4 antibody, rabbit anti-Histone H3 K4 me1 antibody, H3 K4me1, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782
Note	Anti-Histone H3 [Monomethyl Lys4] antibody has been tested in ELISA, Dot blot, and Western Blot. Histone3 K4me1 is useful for Western Blot, Immunocytochemistry, Immunofluorescence, Chromatin Immunoprecipitation, and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in the appropriate cell lysate or extract.
Reference Data

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