PRXC, HRPC Mouse Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	WB: 1:500 - 1:3,000 IHC: User Optimized ELISA: 1:1,000 - 1:5,000
Host	Mouse
Clonality	Polyclonal
Immunogen	Peroxidase [Horseradish]
Specificity	Anti-Peroxidase (horseradish) (Mouse) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum as well as purified and partially purified Peroxidase [Horseradish]. Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration	1.0mg/mL - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Background	Horse Radish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Peroxidase (Horseradish) (Mouse) Antibody is ideal for investigators in Microbiology, Immunology, and Cell Biology research.
Synonyms	Mouse anti-peroxidase antibody, mouse anti-HRP, Horseradish peroxidase
Note	Anti-Peroxidase (Horseradish) (Mouse) antibody has been tested by ELISA and western blot and is suitable for dot blot, immunoprecipitation, conjugation, and most immunological methods requiring high titer and specificity.
Reference Data

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