Mouse Monoclonal Antibody [Clone ID: 8B9.C6.D3]

Specifications

Product Data
Clone Name	8B9.C6.D3
Applications	ELISA, FC, IHC, WB
Recommend Dilution	WB: 1:500 - 1:3,000 IHC: 1:250 - 1:1,500 FC: 1-2 µg/mL ELISA: 1:5,000 - 1:30,000
Host	Mouse
Clonality	Monoclonal
Immunogen	Fluorescein conjugated to Mouse IgG
Specificity	Fluorescein Antibody was prepared from mouse ascites by Protein A/G chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Fluorescein conjugated IgG and Fluorescein conjugated Bovine Serum Albumin.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	0.51mg/mL - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Background	Anti-Fluorescein can be used as visualization reagent for fluorescein-labelled compounds (such as antibodies, lectins and peptide nucleic acid (PNA) probes). Anti-FITC is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting.
Synonyms	mouse Anti-FITC antibody, mouse anti-fluorescein antibody
Note	Anti-Fluorescein Antibody has been tested by ELISA, western blot, and immunofluorescence. This product is suitable for immunomicroscopy, in situ hybridization and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.