zwf Goat Polyclonal Antibody
CAT#: TA396733
Glucose-6-Phosphate Dehydrogenase Antibody Biotin Conjugated
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CNY 7,688.00
货期*
4周
规格
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Specifications
Product Data | |
Applications | ELISA, WB |
Recommend Dilution | WB: 1:500 - 1:2,000 ELISA: 1:5,000 - 1:20,000 |
Reactivity | Leuconostoc mesenteroides |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Glucose-6-Phosphate Dehydrogenase [Leuconostoc mesenteroides] |
Specificity | Anti-Glucose-6-Phosphate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glucose-6-Phosphate Dehydrogenase [Leuconostoc mesenteroides]. Cross reactivity against Glucose-6-Phosphate Dehydrogenase from other sources may occur but have not been specifically determined. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL |
Concentration | 1.0 mg/mL - lot specific |
Conjugation | Biotin |
Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Database Link | |
Background | Anti-Glucose-6-Phosphate Dehydrogenase recognizes the oxidoreductase glucose-6-phosphate dehydrogenase. Found in the cytosol, glucose-6-phosphate dehydrogenase is responsible for oxidizing glucose-6-phosphate and reducing NADP to NADPH as part of the pentose phosphate pathway. As such, glucose-6-phosphate dehydrogenase is crucial in the maintenance of NADPH levels. A deficiency of glucose-6-phosphate dehydrogenase is a risk factor for non-immune hemolytic anemia. Glucose-6-phosphate dehydrogenase may also play a role in cell growth and proliferation and therefore, cancer. |
Synonyms | goat anti-Glucose-6-Phosphate Dehydrogenase Antibody biotin Conjugation, biotin Conjugated goat anti-Glucose-6-Phosphate Dehydrogenase Antibody, G6PD antibody, G6PD1 antibody, G6pdx antibody, Glucose 6 phosphate 1 dehydrogenase antibody, MET19 antibody, POS10 antibody, Zwf1p antibody |
Note | Anti-Glucose-6-Phosphate Dehydrogenase Biotin has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Glucose-6-Phosphate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. |
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