ALDOA Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	WB: 1:500 - 1:5,000 ELISA: 1:1,000 - 1:4,000
Reactivity	Human, Rabbit
Host	Goat
Clonality	Polyclonal
Immunogen	Aldolase [Rabbit Muscle]
Specificity	Anti-Aldolase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Aldolase [Rabbit Muscle].  Cross reactivity against Aldolase from other sources may occur but have not been specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	Biotin
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	Entrez Gene 100009055 Rabbit UniProt ID P00883
Background	Part of the class I fructose-bisphosphate aldolase family, the Anti-Aldolase antibody is essential in the processes glycolysis and gluconeogenesis, as well as performing the role of a scaffolding protein. Anti-Aldolase antibody is ideal for investigators interested in Metabolism, Cancer, and Signal Transduction research.
Synonyms	goat anti-Aldolase Antibody, biotin conjugated goat anti-Aldolase Antibody, Fructose-bisphosphate aldolase A, Muscle-type aldolase
Note	Anti-Aldolase Biotin has been tested by ELISA, immunoprecipitation, and western blot. This product is assayed against 1.0 ug of Aldolase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for this product.
Reference Data

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