LAF Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	WB: 1:500 - 1:5,000 ELISA: 1:5,000 - 1:20,000
Reactivity	Bacterial
Host	Goat
Clonality	Polyclonal
Immunogen	Maltose Phosphorylase [E.coli]
Specificity	Anti-Maltose Phosphorylase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Maltose Phosphorylase [E.coli].  Cross reactivity against Maltose Phosphorylase from other sources is unknown.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	Biotin
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	UniProt ID B2GEX2
Background	Anti-Maltose Phosphorylase recognizes the protein maltose phosphorylase which belongs to the glycosyltransferases family. Maltose phosphorylase catalyzes the reaction in which maltose and inorganic phosphate are converted to D-glucose and beta-D-glucose 1-phosphate.
Synonyms	goat anti-Maltose Phosphorylase Antibody biotin Conjugation, Biotin Conjugated goat anti-Maltose Phosphorylase Antibody, mapA antibody, mpA antibody
Note	Anti-Maltose Phosphorylase antibody has been assayed against 1.0 µg of Maltose Phosphorylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Reference Data

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