gdhB Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1:500 - 1:3,000 ELISA: 1:5,000 - 1:25,000
Reactivity	Bacillus species
Host	Goat
Clonality	Polyclonal
Immunogen	Glucose Dehydrogenase [Bacillus species]
Specificity	Anti-Glucose Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glucose Dehydrogenase [Bacillus species].  Cross reactivity against Glucose Dehydrogenase from other sources is unknown.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration	1.0 mg/mL - lot specific
Conjugation	Biotin
Storage Condition	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Database Link	UniProt ID P07999
Background	Anti-Glucose Dehydrogenase antibody recognizes glucose dehydrogenase. A member of the oxidoreductase family that acts on CH-OH group on donors, glucose dehydrogenase uses NAD+ or NADP+ as the acceptor. Glucose dehydrogenase reacts with beta-D-glucose to produce D-glucono-1,5-lactone and depending on the acceptor, either NADH or NADPH. Anti-Glucose Dehydrogenase is ideal for investigators interested in Metabolism, Cancer, and Signal Transduction.
Synonyms	goat anti-Glucose Dehydrogenase Antibody biotin Conjugation, biotin Conjugated goat anti-Glucose Dehydrogenase Antibody, gdh antibody, gdh2 antibody, gdh3 antibody, gdh4 antibody, gdhI antibody, GLCDHIII antibody, GLCDHIV antibody, Glucose 1 dehydrogenase 1 antibody
Note	Anti-Glucose Dehydrogenase has been assayed against 1.0 ug of Glucose Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Reference Data

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