BLRO_MYRVE Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	WB: 1:500 - 1:2,500 ELISA: 1:1,000 - 1:5,000
Reactivity	Myrothecium verrucaria
Host	Goat
Clonality	Polyclonal
Immunogen	Bilirubin Oxidase [Myrothecium verrucaria]
Specificity	Anti-Bilirubin Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Bilirubin Oxidase [Myrothecium verrucaria].  Cross reactivity against Bilirubin Oxidase from other sources is unknown.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	Biotin
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	UniProt ID Q12737
Background	Anti-Bilirubin Oxidase antibody is an enzyme that increases the oxidation rate of bilirubin to biliverdin and other substrates. It is ideal for investigators interested in Metabolism or Signal Transduction research.
Synonyms	goat anti-Bilirubin Oxidase biotin conjugation, goat anti-Bilirubin Oxidase Antibody biotin Conjugated
Note	Anti-Bilirubin Oxidase has been tested by western blot and is suitable to be assayed against 1.0 ug of Bilirubin Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.
Reference Data

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