glpK Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1:500 - 1:2,500 ELISA: 1:1,000 - 1:5,000
Host	Goat
Clonality	Polyclonal
Immunogen	Glycerol Kinase [Cellulomonas species]
Specificity	Anti-Glycerol Kinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glycerol Kinase [Cellulomonas species].  Cross reactivity against Glycerol Kinase from other sources is unknown.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration	1.0 mg/mL - lot specific
Conjugation	Biotin
Storage Condition	Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Database Link	UniProt ID D0VZG4
Background	Anti-Glycerol Kinase antibody recognizes the phosphotransferase enzyme glycerol kinase. Glycerol kinase catalyzes the formation of glycerol phosphate by transferring a phosphate from ATP to glycerol. Dihydroxyacetone and L-glyceraldehyde can also act as acceptors in place of glycerol. Anti-Glycerol Kinase is ideal for investigators interested in Cancer, Metabolism, and Kinase/Phosphatase enzymology.
Synonyms	goat anti-Glycerol Kinase Antibody biotin Conjugation, biotin Conjugated goat anti-Glycerol Kinase Antibody, ATP glycerol 3 phosphotransferase antibody, GK antibody, GK antibody, GK1 antibody, GKD antibody, Glycerokinase antibody, Glycerol kinase antibody, Glycerol kinase deficiency antibody
Note	Anti-Glycerol Kinase has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Glycerol Kinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Reference Data

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