Rabbit Polyclonal Antibody

CAT#: TA396534

E.coli HCP Antibody



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CNY 8,025.00


货期*
4周

规格
    • 100 ul

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Specifications

Product Data
Applications ELISA, WB
Recommend Dilution WB: 1:500 - 1:1000
ELISA: 1:1000 - 1:2000
Reactivity Escherichia coli
Host Rabbit
Clonality Polyclonal
Immunogen Anti-Host Cell Proteins Antibody was prepared blending antibodies that have been separately developed by repeated immunizations with a mixture of high molecular weight (HMW) and a mixture of low molecular weight (LMW) proteins from E.Coli (BL21).
Specificity E.coli HCP Antibody is directed against high molecular weight and low molecular weight E.coli Host Cell Proteins. This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. The anti-sera reactivity is directed against E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration 77.5 mg/mL - lot specific
Conjugation Unconjugated
Storage Condition Store Host Cell Proteins Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Background Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli. This antibody has been validated by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.
Synonyms rabbit anti-E.Coli Host Cell Protein antibody, HCP antibody, Anti-HCP, Anti-Host Cell Protein, ECP antibody, Anti-ECP, 2D detection antibody
Note Anti-Host Cell Proteins Antibody detects over 300 E.coli proteins and has been tested in ELISA, 2D-Page and Western blot.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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