RAD21 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:500~1:1000 IHC: 1:50~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 521-570 of Human Rad21.
Specificity	Rad21 (D548) polyclonal antibody detects endogenous levels of Rad21 protein.
Formulation	Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Concentration	1mg/ml
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Predicted Protein Size	~ 130 kDa
Gene Name	RAD21 cohesin complex component
Database Link	Entrez Gene 5885 Human UniProt ID O60216
Background	Rad21 is one of the major cohesin subunits that holds sister chromatids together until anaphase, when proteolytic cleavage by separase, a caspaselike enzyme, allows chromosomal separation. Rad21 interacts with Rec8 to form a cohesin complex that functions in sister chromatid alignment. Rad21 is also involved in the repair of double-strand breaks in DNA and is essential for mitotic growth. Rad21 undergoes a C-terminal cleavage induced by diverse stimuli right before apoptosis. The cleavage product migrates to the cytoplasm and is involved in early events in the apoptotic pathway and it amplifiies the cell death signal in a positive-feedback manner.
Synonyms	Double-strand-break repair protein rad21 homolog; hHR21; HR21; KIAA0078; Nuclear matrix protein 1; NXP-1; NXP1; RAD21; SCC1 homolog
Note	For research use only, not for use in diagnostic procedure.
Reference Data

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