Thyroid Hormone Receptor alpha (THRA) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	WB: 1:2000~1:5000 IF: 1:50~1:200
Reactivity	Human, Pig
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to Human TRα.
Specificity	TRα polyclonal antibody detects endogenous levels of TRα protein.
Formulation	Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Concentration	1mg/ml
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Predicted Protein Size	~ 58 kDa
Gene Name	thyroid hormone receptor, alpha
Database Link	Entrez Gene 7067 Human UniProt ID P10827
Background	Thyroid hormone nuclear receptors (TRs) are ligand-dependent transcription factors which regulate and control many metabolic and developmental processes. There are two genes encoding TRs identified to date, TRα and TRβ. TRs bind to thyroid hormone response elements (TREs) with half-site binding motifs in the orientation of palindromes, direct repeats or inverted palindromes. The affinities of binding are both variable and influenced differentially by 3,5,3'-triiodo-L-thyronine (T3). Transcriptional regulation by TRs is also modulated by heterodimerization with TR nuclear accessory proteins, the most extensively characterized of which are the retinoid X receptors (RXRα, RXRβ and RXRγ). The TRα isoform TRα1 can display both a nuclear and undefined cytoplasmic location, and is the only TR that is imported into the mitochondrial matrix. TRα2 is a C-terminal variant of TRα1 that does not bind thyroid hormones (THs) and weakly binds DNA. TRα2 acts as a dominant negative antagonist of TH signalling.
Synonyms	c-erbA-1; c-erbA-alpha; EAR-7; Nuclear receptor subfamily 1 group A member 1; Thyroid hormone receptor alpha; V-erbA-related protein 7
Note	For research use only, not for use in diagnostic procedure.
Reference Data

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