VASP Mouse Antibody [Clone ID: M277]

Specifications

Product Data
Clone Name	M277
Applications	ICC, WB
Recommend Dilution	WB: 1:1000 ICC: 1:50
Reactivity	Human
Host	Mouse
Immunogen	Clone (M277) was generated from a recombinant protein that includes amino acids from the C-terminal region of human VASP.
Specificity	This antibody detects 46 and 50 kDa* proteins corresponding to the apparent molecular mass of VASP on SDS-PAGE immunoblots of human A431, HeLa, and HUVEC.
Formulation	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Concentration	lot specific
Purification	Protein A Purified
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	46/50
Database Link	UniProt ID P50552
Background	Actin filament tethering and bundling are important mechanisms involved in actin superstructure assembly. The ENA/VASP family includes VASP, mena, and Ena-Vasp-like (EVL). These multidomain proteins localize to the leading edge of filopodia where they associate with AFs, interact with profilin, and compete with capping proteins at the barbed end of AFs. Artificial relocalization of VASP from the plasma membrane to mitochondrial membranes inhibits filopodial formation and axon branching, while deletion of all three ENA/VASP proteins produces defects in cortical axon-tract formation. Regulation of VASP protein activity occurs through phosphorylation at Ser-157, Ser-239, and Thr-278. AMPK phosphorylates Thr-278, leading to impaired actin stress fiber assembly and changes in cell morphology.
Note	Protein G purified tissue culture supernatant.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.