TRPM7 Mouse Antibody [Clone ID: M573]

Specifications

Product Data
Clone Name	M573
Applications	WB
Recommend Dilution	WB: 1:500
Reactivity	Human, Rat, Mouse
Host	Mouse
Immunogen	Clone M573 was generated from TRPM7 synthetic peptide (coupled to carrier) corresponding to amino acids in the extracellular region of human TRPM7. This site is well conserved in rat and mouse TRPM7, but has low homology to other TRPM family members.
Specificity	This antibody detects a 220 kDa* protein on SDS-PAGE immunoblots of human MCF7 cells.
Formulation	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Concentration	lot specific
Purification	Antigen Affinity Purified
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	220
Database Link	UniProt ID Q96QT4
Background	The transient receptor potential melastatin (TRPM) subfamily of cation-permeable TRP channels is ubiquitously expressed in mammalian tissues. This family includes TRPM1-8. In addition to acting as a calcium-permeant channel, TRPM6 and TRPM7 possess an inherent serine/threonine kinase activity. TRPM7 specifically is involved with cellular magnesium homeostasis and neurotransmitter release. Due to the magnesium inhibition, TRPM7’s ion channel activity is very low. TRPM7 has been implicated in cell proliferation and migration during cancer progression, and its expression levels correlate with prognosis in breast cancer. TRPM7 kinase activation leads to massive autophosphorylation of the C-terminal region, including phosphorylation of Ser-1493, Ser-1513, and Ser-1569. Both Ser-1513 and Ser-1569 phosphorylation is required for kinase activity, and phosphorylation of Ser-1513 may inhibit Caspase-mediated cleavage of the C-terminal tail. Thus, TRPM7 is a multifunctional transmembrane protein with roles in cell signaling, proliferation, migration, and death.
Note	Protein G purified tissue culture supernatant.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.