ITGB1 Mouse Antibody [Clone ID: M032]

Specifications

Product Data
Clone Name	M032
Applications	ICC, WB
Recommend Dilution	WB: 1:500 ICC: 1:200
Reactivity	Human
Host	Mouse
Immunogen	Clone M032 was generated from a proprietary antigen related to the extracellular region of human integrin β1 expressed in A431 epidermoid carcinoma cell line.
Specificity	Clone M032 detects a 120-150 kDa* band corresponding to Integrin β1 on SDS-PAGE immunoblots of "Native" human A431, A549, MDA-MB-231, and LNCaP cell lysates, as well as human breast, skin, and lung tissues. The antibody weakly detects the denatured form of Integrin β1. Clone M032 can be used in western blot, immunocytochemistry, and ELISA.
Formulation	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Concentration	lot specific
Purification	Protein G Purified
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	120-150
Database Link	UniProt ID P05556
Background	Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. The integrin β2 subunit associates with integrin αL to form a receptor for ICAM family members. Integrin β2/αL is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.
Note	Protein G purified tissue culture supernatant.
Reference Data

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