EPHA4 Mouse Antibody [Clone ID: M280]

Specifications

Product Data
Clone Name	M280
Applications	WB
Recommend Dilution	WB: 1:1000
Reactivity	Human, Rat, Mouse, Chicken
Host	Mouse
Immunogen	Clone M280 was generated from a recombinant protein corresponding to amino acids in the N-terminal region of human EphA4. This region has less than 50% homology to similar regions in other EphA and EphB family members.
Specificity	This antibody detects 95 and 120 kDa* bands corresponding to EphA4 in Western blots of HUVEC cells and a 120 kDa band in mouse brain tissue.
Formulation	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Concentration	lot specific
Purification	Protein A Purified
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	95/120
Database Link	UniProt ID P54764
Background	The Eph family of Receptor tyrosine kinases and their Ephrin ligands are important for cell positioning and morphogenesis during development. Eph receptors are classified into 10 EphA and 6 EphB receptors, which preferentially bind to the type A and type B ephrins, respectively. The EphA4 receptor can inhibit axon outgrowth and has roles in regulating axon projections during neural development. EphA4 signaling pathways require its kinase activity and involve binding and activation of Rho-GTPase guanine nucleotide-exchange factors (GEFs). EphA4 activation leads autophosphorylation of Tyr-596 and Tyr-602, and the conserved sites in EphA2 are required for binding to the GEFs, Vav2 and Vav3, and ephrin-induced cell migration. The Tyr-779 site in the kinase domain is also phosphorylated in vivo and may regulate kinase activity. Activated EphA4 leads to Src kinase phosphorylation of the GEF, ephexin-1, and this activates RhoA. Thus, EphA4 signaling involves complex tyrosine phosphorylation in its cytoplasmic region along with interaction with several GEFs.
Note	Protein G purified tissue culture supernatant.
Reference Data

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