CASP3 Mouse Antibody [Clone ID: M377]

Specifications

Product Data
Clone Name	M377
Applications	ICC, WB
Recommend Dilution	WB: 1:1000 ICC: 1:100
Reactivity	Human
Host	Mouse
Immunogen	Clone (M377) was generated from a recombinant protein containing amino acid residues in the N-terminal region of human Caspase-3.
Specificity	The antibody detects a 32 kDa* protein corresponding to the molecular weight of Caspase-3 on SDS-PAGE immunoblots of human Jurkat and K562 cells.
Formulation	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Concentration	lot specific
Purification	Protein A Purified
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	32
Database Link	UniProt ID P42574
Background	The caspases are a group of cysteine enzymes, which cleave proteins in response to intrinsic and extrinsic pathways that cause apoptotic cell death. The caspases can be grouped into two subgroups based on their roles in apoptosis. Initiator caspases (caspases 2, 8, 9, and 10) are activated through the apoptosis-signaling pathways and activate the effector caspases (caspases 3, 6, and 7) which carry out apoptosis. Caspase cascades are initiated through assembly of multiprotein complexes that trigger activation of the initiator caspases, which are then released and are able to activate the downstream effector caspases.
Note	Protein G purified tissue culture supernatant.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.