S Mouse Monoclonal Antibody [Clone ID: 5G8]

Specifications

Product Data
Clone Name	5G8
Applications	ICC, WB
Recommend Dilution	WB: 1:2000 ICC: 1:1000
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant SARS-CoV-2 S-Protein construct containing the ACE2 binding domain expressed in and purified from E. coli.
Specificity	Specific for the ~25 kDa ACE2 binding domain construct of SARS-CoV-2 S protein. Full-length CoV-2 S protein is ~142 kDa.
Formulation	PBS + 50% glycerol and 5 mm NaN3
Concentration	lot specific
Purification	Protein G Purified
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	25 or 142 full-length
Background	The novel SARS-coronavirus 2 (SARS-CoV-2) which causes the disease COVID-19 has been shown to utilize the SARS-CoV receptor ACE2 for entry into human cells (Hoffman, M. et al., Cell 2020). The entry of a coronavirus into host cells is mediated by the viral surface-anchored transmembrane spike (S) glycoprotein which is composed of two functional subunits, S1 which binds the receptor and S2 which fuses the membrane (Walls, AC et.al., Cell 2020). S1 contains a receptor-binding domain (RBD) which specifically recognizes ACE2 as its receptor (Wan, Y. et al., 2020).
Note	Protein G purified culture supernatant.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.