Mouse Monoclonal Antibody [Clone ID: 6F7]

Specifications

Product Data
Clone Name	6F7
Applications	ICC, IHC, WB
Recommend Dilution	WB: 1:1000 ICC: 1:500-1:5000
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant protein from C-terminal region of Cas9 from Staphylococcus aureus.
Specificity	Specific for the Staphylococcus aureus Cas9 protein.
Formulation	PBS + 50% glycerol and 5 mm NaN3
Concentration	lot specific
Purification	Protein G Purified
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	53
Background	CRISPR/Cas9 is a genome editing tool that makes possible the permanent alteration of genes by directing specifically designed RNA sequences complexed with the nuclease Cas9 to cleave DNA at any desired location allowing the deletion or addition of genes. Antibodies against Cas9 are very useful in the direct detection of the CRISPR/Cas9 complex. This antibody detects Cas9 from Staphylococcus aureus transfected into cells and shows no reactivity toward the Streptococcus pyogenes Cas9 protein (Ran et al., 2015).
Note	Protein G purified culture supernatant.
Reference Data

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