ACTN4 Mouse Monoclonal Antibody [Clone ID: 93]

CAT#: TA388989

Anti-Alpha Actinin 4 Antibody



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CNY 5,325.00


货期*
5周

规格
    • 100 ul

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Specifications

Product Data
Clone Name 93
Applications IHC, WB
Recommend Dilution WB: 1:1000
WB Brain: 1:1000
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Immunogen Fusion protein from the central rod domain of human α-actinin 4.
Specificity Specific for endogenous levels of the ~105 kDa α-actinin 4 protein.
Formulation 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Concentration lot specific
Purification Protein G purified
Conjugation Unconjugated
Storage Condition Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size 105
Background α-actinin-4 is a member of the actinin protein family comprised of an actin-binding domain in the N-terminus, 4 spectrin-like repeats in the central region, and 2 EF-hand motifs in the C-terminus (Honda et al, 1998). α-actinin-4 and CLP36 form a complex in normal kidney podocytes.CLP36 is dependent on α-actinin-4 for maintenance of its level in podocytes, whereas α-actinin-4 is independent of CLP36. α-actinin-4 is widely expressed in mammalian tissues and organs, while having a high occurrence of genetic mutations in kidney podocytes (Kos et al, 2003). FSGS, focal segmental glomerulosclerosis, is a rare genetic disease that attacks the kidney's filtering units (glomeruli) causing serious scarring which leads to permanent kidney damage and even failure. Three key mutations have been found in α-actinin-4 in people diagnosed with FSGS. R310Q and Q348R, located in the spectrin-like repeats region, and K255E located in the actin-binding region. The R310Q and Q348R mutation significantly inhibits the ability of α-actinin-4 to form the complex with CLP36. The K255E mutation was reversed where it increased the ability to bind CLP36 in the actin-binding region (Liu et al, 2011).
Note Protein G purified cultured supernatant.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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