Cxcl12 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Sandwich ELISA: To detect Rat SDF-1 alpha by sandwich ELISA (using 100ul/well antibody solution), a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Rat SDF-1 alpha as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat SDF-1 alpha. Western Blot To detect Rat SDF-1 alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat SDF-1 alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Produced from sera of rabbits immunized with highly pure recombinant Rat SDF-1 alpha. Anti-Rat SDF-1 alpha-specific antibody was purified by affinity chromatography employing an immobilized Rat SDF-1 alpha matrix.
Concentration	lot specific
Purification	SDF-1 alpha-specific antibody was purified by affinity chromatography employing an immobilized Rat SDF-1 alpha matrix
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Background	SDF-1 alpha andbeta are stromal-derived, CXC chemokines that signal through the CXCR4 receptor. SDF-1alpha andbeta chemoattract B and T cells, and have been shown to induce migration of CD34+ stem cells. Additionally, the SDF-1 proteins exert HIV-suppressive activity in cells expressing the CXCR4 receptor. Human and murine SDF-1 proteins act across species. SDF-1 alpha andbeta contain the four highly conserved cysteine residues present in CXC chemokines. The mature SDF-1 alpha protein is the result of alternative splicing of the SDF-1 gene and contains 68 amino acid residues. Recombinant Rat SDF-1alpha is a 7.9 kDa protein containing 68 amino acid residues.
Reference Data

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