Cxcl12 Rabbit Polyclonal Antibody
CNY 7,481.00
货期*
5周
规格
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Specifications
Product Data | |
Applications | ELISA, WB |
Recommend Dilution | Sandwich ELISA: To detect Rat SDF-1 alpha by sandwich ELISA (using 100ul/well antibody solution), a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Rat SDF-1 alpha as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat SDF-1 alpha. Western Blot To detect Rat SDF-1 alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat SDF-1 alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivity | Rat |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Produced from sera of rabbits immunized with highly pure recombinant Rat SDF-1 alpha. Anti-Rat SDF-1 alpha-specific antibody was purified by affinity chromatography employing an immobilized Rat SDF-1 alpha matrix. |
Concentration | lot specific |
Purification | SDF-1 alpha-specific antibody was purified by affinity chromatography employing an immobilized Rat SDF-1 alpha matrix |
Conjugation | Unconjugated |
Storage Condition | Store at -20°C as received. |
Background | SDF-1 alpha andbeta are stromal-derived, CXC chemokines that signal through the CXCR4 receptor. SDF-1alpha andbeta chemoattract B and T cells, and have been shown to induce migration of CD34+ stem cells. Additionally, the SDF-1 proteins exert HIV-suppressive activity in cells expressing the CXCR4 receptor. Human and murine SDF-1 proteins act across species. SDF-1 alpha andbeta contain the four highly conserved cysteine residues present in CXC chemokines. The mature SDF-1 alpha protein is the result of alternative splicing of the SDF-1 gene and contains 68 amino acid residues. Recombinant Rat SDF-1alpha is a 7.9 kDa protein containing 68 amino acid residues. |
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