PD-L1 (CD274) Rabbit Monoclonal Antibody [Clone ID: 332M7]

Specifications

Product Data
Clone Name	332M7
Applications	Bl, FC
Reactivity	Human, Mouse, Monkey
Host	Rabbit
Clonality	Monoclonal
Immunogen	The original antibody was generated by immunizing BALB/c mice with recombinant human PD-L1.
Specificity	This antibody strongly binds human and cynomolgus monkey PD-L1 and shows weak binding for mouse PD-L1. PD-L1 is highly expressed in many human tumors. . PD-L1 exerts an immune suppressive signal through binding to PD-1 and B7.1, and tumor expression of PD-L1 can mediate cancer immune evasion. Blocking this pathway, therefore provides a rationale for cancer immunotherapy. The binding characterization of this antibody towards human, mouse and cynomolgus monkey PD-L1 was done using flow cytometry. This antibody was shown to block the binding of hPD-1-Fc protein to hPD-L1 expressed on the surface of the HEK-293T cells at a concentration of 10 μg/ml. The mouse version of this antibody was reported to inhibit patient derived melanoma tumor in tumor-bearing mice. There was also found an increase in the gene expression of CD8 and IFN-γ in tumor samples from mice treated with mouse version of this antibody (US20180346571).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	CD274 molecule
Database Link	Entrez Gene 29126 Human UniProt ID Q9NZQ7
Synonyms	B7-H; B7-H1; B7H1; MGC142294; MGC142296; PD-L1; PDCD1L1; PDCD1LG1; PDL1
Note	This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

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